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Long‐term potentiation and long‐term depression induced by local application of ATP to hippocampal CA1 neurons of the Guinea pig
Author(s) -
Yamazaki Yoshihiko,
Kaneko Kenya,
Fujii Satoshi,
Kato Hiroshi,
Ito KenIchi
Publication year - 2002
Publication title -
hippocampus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.767
H-Index - 155
eISSN - 1098-1063
pISSN - 1050-9631
DOI - 10.1002/hipo.7999
Subject(s) - schaffer collateral , long term potentiation , excitatory postsynaptic potential , hippocampal formation , neuroscience , hippocampus , chemistry , synaptic plasticity , postsynaptic current , postsynaptic potential , biophysics , ltp induction , inhibitory postsynaptic potential , biology , biochemistry , receptor
Abstract The present study has investigated the role of ATP in the induction of synaptic plasticity, using local application of ATP by picopump administration into the stratum radiatum of guinea pig hippocampal region CA1. Excitatory postsynaptic currents (EPSCs) evoked by stimulation of Schaffer collateral/commissural afferents synapsing on CA1 pyramidal cells of hippocampal slices were monitored in voltage‐clamp mode, using whole‐cell recording. Brief local application of ATP (1 mM) induced an inward current, usually consisting of early‐ and late‐phase components. Because the late‐phase component of an ATP‐induced current was largely inhibited by Ca 2+ ‐free solution, this component is supposed to depend on extracellular Ca 2+ . After local application of ATP, long‐term synaptic modification of EPSCs was induced: LTP was detected in neurons exhibiting a small late Ca 2+ current, while LTD was obtained from recordings showing a large late Ca 2+ current in response to ATP application. There was a statistically significant correlation between the magnitude of long‐term plastic changes and the size of Ca 2+ currents in response to ATP application. Furthermore, there was significant difference between the average size of the Ca 2+ current in the LTP group and the size in the LTD group. These results suggest that a small Ca 2+ influx in response to ATP application induces LTP, whereas a large one induces LTD in guinea pig hippocampal CA1 neurons. Hippocampus 2003;13:81–92. © 2003 Wiley‐Liss, Inc.