Premium
Transport of cytoskeletal proteins in axons of hippocampal pyramidal cells
Author(s) -
Watson Danny F.,
Fittro Kenneth P.
Publication year - 1993
Publication title -
hippocampus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.767
H-Index - 155
eISSN - 1098-1063
pISSN - 1050-9631
DOI - 10.1002/hipo.450030413
Subject(s) - cytoskeleton , hippocampal formation , axoplasmic transport , tubulin , microtubule , chemistry , neurofilament , microbiology and biotechnology , fornix , neuroscience , hippocampus , biology , cell , biochemistry , immunohistochemistry , immunology
Axonal transport of cytoskeletal proteins has not yet been extensively studied in the brain proper, in contrast to the peripheral nerves and optic nerves. The authors have developed a means for the study of transport of cytoskeletal proteins in axons of hippocampal pyramidal cells. Proteins of intrinsic neurons of the dorsal hippocampus were labeled by microinjection of 35 S methionine, and the subsequent transport of labeled proteins was characterized in the axons projecting into the fimbria‐fornix. A peak of labeled proteins was present in the fimbria‐fornix at 4–12 days after labeling, corresponding to transport rates 0.2–0.7 mm/day. The most abudant proteins at each time studied exhibited one‐dimensional electrophoretic mobilites of actin and tubulin; neurofilaments were less intensely labeled. The observed specializations of cytoskeletal transport, especially the paucity of tubulin transport at rates of 2–4 mm/day, may predispose hippocampal pyramidal cells to accumulate tubulin and microtubule‐associated proteins in their cell bodies in various disease states.