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The electrophysiology of the olfactory–hippocampal circuit in the isolated and perfused adult mammalian brain in vitro
Author(s) -
de Curtis Marco,
Paré Denis,
Lliás Rodolfo R.
Publication year - 1991
Publication title -
hippocampus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.767
H-Index - 155
eISSN - 1098-1063
pISSN - 1050-9631
DOI - 10.1002/hipo.450010402
Subject(s) - neuroscience , hippocampal formation , electrophysiology , limbic system , entorhinal cortex , isolated brain , hippocampus , slice preparation , chemistry , biology , central nervous system
The viability and general electrophysiological properties of the limbic system in the adult mammalian brain isolated and maintained in vitro by arterial perfusion are described. The isolated brain preparation combines the advantages of intact synaptic connectivity and accessibility of different areas of the encephalic mass with those of the in vitro approach, i.e., stability and control of the ionic environment. Extracellular field potential as well as intracellular recordings were performed at different levels in the limbic system of isolated adult guinea pig brains. The results demonstrate that in the piriform, entorhinal, and hippocampal cortices, the intrinsic electrical properties of individual cells as well as the spontaneous and evoked electrical activity in the neuronal ensembles they comprise, were virtually identical to those observed in vivo . The properties of the limbic system loop were determined.