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Involvement of intracellular Zn 2+ signaling in LTP at perforant pathway–CA1 pyramidal cell synapse
Author(s) -
Tamano Haruna,
Nishio Ryusuke,
Takeda Atsushi
Publication year - 2017
Publication title -
hippocampus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.767
H-Index - 155
eISSN - 1098-1063
pISSN - 1050-9631
DOI - 10.1002/hipo.22730
Subject(s) - long term potentiation , intracellular , chemistry , perforant pathway , pyramidal cell , neuroscience , extracellular , perforant path , synapse , synaptic plasticity , schaffer collateral , postsynaptic potential , hippocampal formation , biophysics , microbiology and biotechnology , biology , biochemistry , receptor
Physiological significance of synaptic Zn 2+ signaling was examined at perforant pathway–CA1 pyramidal cell synapses. In vivo long‐term potentiation (LTP) at perforant pathway–CA1 pyramidal cell synapses was induced using a recording electrode attached to a microdialysis probe and the recording region was locally perfused with artificial cerebrospinal fluid (ACSF) via the microdialysis probe. Perforant pathway LTP was not attenuated under perfusion with CaEDTA (10 mM), an extracellular Zn 2+ chelator, but attenuated under perfusion with ZnAF‐2DA (50 μM), an intracellular Zn 2+ chelator, suggesting that intracellular Zn 2+ signaling is required for perforant pathway LTP. Even in rat brain slices bathed in CaEDTA in ACSF, intracellular Zn 2+ level, which was measured with intracellular ZnAF‐2, was increased in the stratum lacunosum‐moleculare where perforant pathway–CA1 pyramidal cell synapses were contained after tetanic stimulation. These results suggest that intracellular Zn 2+ signaling, which originates in internal stores/proteins, is involved in LTP at perforant pathway–CA1 pyramidal cell synapses. Because the influx of extracellular Zn 2+ , which originates in presynaptic Zn 2+ release, is involved in LTP at Schaffer collateral‐CA1 pyramidal cell synapses, synapse‐dependent Zn 2+ dynamics may be involved in plasticity of postsynaptic CA1 pyramidal cells.