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Time‐dependent changes in the mouse hippocampal synaptic membrane proteome after contextual fear conditioning
Author(s) -
RaoRuiz Priyanka,
Carney Karen E.,
Pandya Nikhil,
van der Loo Rolinka J.,
Verheijen Mark H.G.,
van Nierop Pim,
Smit August B.,
Spijker Sabine
Publication year - 2015
Publication title -
hippocampus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.767
H-Index - 155
eISSN - 1098-1063
pISSN - 1050-9631
DOI - 10.1002/hipo.22432
Subject(s) - hippocampal formation , neuroscience , fear conditioning , hippocampus , nmda receptor , glutamate receptor , ampa receptor , chemistry , biology , psychology , biochemistry , receptor , amygdala
A change in efficacy of hippocampal synapses is critical for memory formation. So far, the molecular analysis of synapses during learning has focused on small groups of proteins, whereas the dynamic global changes at these synapses have remained unknown. Here, we analyzed the temporal changes of the mouse hippocampal synaptic membrane proteome 1 and 4 h after contextual fear learning, comparing two groups; (1) a fear memory forming “delayed‐shock” group and (2) a fear memory‐deficient “immediate ‐ shock” group. No changes in protein expression were observed 1 h after conditioning between the two experimental groups. However, 423 proteins were significantly regulated 4 h later of which 164 proteins showed a temporal regulation after a delayed shock and 273 proteins after the stress of an immediate shock. From the proteins that were differentially regulated between the delayed‐ and the immediate ‐ shock groups at 4 h, 48 proteins, most prominently representing endocytosis, (amphiphysin, dynamin, and synaptojanin1), glutamate signaling (glutamate [NMDA] receptor subunit epsilon‐1, disks large homolog 3), and neurotransmitter metabolism (excitatory amino acid transporter 1, excitatory amino acid transporter 2, sodium‐ and chloride‐dependent GABA transporter 3) were regulated in both protocols, but in opposite directions, pointing toward an interaction of learning and stress. Taken together, this data set yields novel insight into diverse and dynamic changes that take place at hippocampal synapses over the time course of contextual fear‐memory learning. © 2015 Wiley Periodicals, Inc.