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Dynamic expression of the polysialyltransferase in adult rat hippocampus performing an olfactory associative task
Author(s) -
Manrique Christine,
Migliorati Martine,
Gilbert Valérie,
Brezun JeanMichel,
Chaillan Franck A.,
Truchet Bruno,
Khrestchatisky Michel,
GuiraudieCapraz Gaëlle,
Roman François S.
Publication year - 2014
Publication title -
hippocampus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.767
H-Index - 155
eISSN - 1098-1063
pISSN - 1050-9631
DOI - 10.1002/hipo.22284
Subject(s) - hippocampus , neuroscience , task (project management) , psychology , olfactory memory , associative property , olfactory system , olfactory bulb , central nervous system , mathematics , management , pure mathematics , economics
Neural cell adhesion molecule (NCAM) is associated with polysialic acid (PSA), and its function is highly dependent on the extent of polysialylation through the activity of two polysialyltransferases, sialyltransferase‐X (STX) and polysialyltransferase (PST). PSA‐NCAM plays an important role in synaptic plasticity in the hippocampus. The involvement of STX and PST during mnesic processes was assessed in the adult rat hippocampus. We investigated whether different levels in learning and memory using an olfactory associative task influenced STX and PST gene expression in the hippocampus using semiquantitative transcription‐polymerase chain reaction. Then, NCAM polysialylation and cell proliferation were quantified in the dentate gyrus of a “Learning and Memory” group using immunohistochemistry. We found that only the expression level of PST mRNA increased with learning performance and returned to an initial level when learned associations were consolidated in long‐term memory, while STX mRNA levels remained unchanged. This phenomenon was accompanied by an increase in PSA on NCAM but not by cell proliferation in the dentate gyrus. Our results suggest a different involvement for STX and PST in neural plasticity: while STX is probably involved in the proliferation of neural progenitor cells, PST could play a key role in synaptic plasticity of mature neural networks. The expression of the STX and PST genes could, therefore, be useful markers of neurobiological plasticity in the brain, allowing to follow chronological events in limbic and cortical structures related first to learning and memory processes (for PST) and, second, to adult neurogenesis processes (for STX). © 2014 Wiley Periodicals, Inc.