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Aberrant hippocampal spine morphology and impaired memory formation in neuronal platelet‐derived growth factor beta‐receptor lacking mice
Author(s) -
Shioda Norifumi,
Moriguchi Shigeki,
Oya Takeshi,
Ishii Yoko,
Shen Jie,
Matsushima Takako,
Nishijo Hisao,
Sasahara Masakiyo,
Fukunaga Kohji
Publication year - 2012
Publication title -
hippocampus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.767
H-Index - 155
eISSN - 1098-1063
pISSN - 1050-9631
DOI - 10.1002/hipo.20973
Subject(s) - synaptophysin , dendritic spine , hippocampal formation , postsynaptic potential , long term potentiation , neuroscience , knockout mouse , hippocampus , postsynaptic density , conditional gene knockout , synaptic plasticity , dendrite (mathematics) , biology , receptor , immunohistochemistry , immunology , phenotype , biochemistry , gene , geometry , mathematics
The physiological role of platelet‐derived growth factor (PDGF) in the central nervous system (CNS) synaptic function remains uncharacterized. Here we identify physiological roles of PDGF receptor‐β (PDGFR‐β) in the CNS by conditional knockout of the gene encoding it. In the hippocampus, PDGFR‐β colocalized immunohistochemically with both presynaptic synaptophysin and postsynaptic density‐95 (PSD‐95). In the hippocampal CA1 region, expression levels of postsynaptic proteins, including spinophilin, drebrin, and PSD‐95, were significantly decreased in PDGFR‐β knockout mice, although presynaptic synaptophysin levels remained comparable to controls. Interestingly, in hippocampal CA1 pyramidal neurons, dendritic spine density in PDGFR‐β knockout mice was significantly decreased compared with that seen in wild‐type mice, although spine length and number of dendritic branches remained unchanged. Consistent with these findings, impairment in hippocampal long‐term potentiation (LTP) and in hippocampus‐dependent memory formation were seen in PDGFR‐β knockout mice. These results suggest PDGFR‐β plays critical roles in spine morphology and memory formation in mouse brain. © 2011 Wiley Periodicals, Inc.