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Pyridoxal‐5′‐phosphate phosphatase/chronophin inhibits long‐term potentiation induction in the rat dentate gyrus
Author(s) -
Kim JiEun,
Kim DaeWon,
Kwak SungEun,
Ryu Hea Jin,
Yeo SeongIl,
Kwon OhShin,
Choi SooYoung,
Kang TaeCheon
Publication year - 2009
Publication title -
hippocampus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.767
H-Index - 155
eISSN - 1098-1063
pISSN - 1050-9631
DOI - 10.1002/hipo.20568
Subject(s) - long term potentiation , dentate gyrus , cofilin , ltp induction , chemistry , phosphatase , microbiology and biotechnology , protein phosphatase 1 , phosphorylation , neuroscience , endocrinology , medicine , hippocampus , actin cytoskeleton , biology , biochemistry , cytoskeleton , receptor , cell
Abstract Pyridoxal‐5′‐phosphate (PLP)‐phosphatase/chronophin (PLPP/CIN) directly dephosphorylates actin‐depolymerizing factor (ADF)/cofilin as well as PLP. Although PLPP/CIN plays a role in the regulation of F‐actin and vitamin B 6 metabolism, there is no direct evidence to support a correlation between PLPP/CIN and F‐actin polymerization during long‐term potentiation (LTP) induction. In this study, we investigated whether the expression of PLPP/CIN is altered following LTP induction, and whether Tat‐PLPP/CIN transduction affects LTP induction in the rat dentate gyrus (DG). PLPP/CIN immunoreactivity was markedly decreased in dentate granule cells after the induction of LTP. Tat‐PLPP/CIN transduction (20 and 200 μg/kg) decreased the efficiency of high frequency stimulus‐induced potentiation of populations spike amplitude as compared to saline or Tat‐protein‐treated animals. The PLPP/CIN protein level showed an inverse correlation with phosphorylated ADF/cofilin levels and F‐actin content. These findings suggest that PLPP/CIN‐mediated actin dynamics may play an important role in the changes of morphological properties (dendritic spine reorganization) of the hippocampus in LTP. © 2009 Wiley‐Liss, Inc.