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Novel recurrent genetic imbalances in human hepatocellular carcinoma cell lines identified by comparative genomic hybridization
Author(s) -
Zimonjic Drazen B.,
Keck Catherine L.,
Thorgeirsson Snorri S.,
Popescu Nicholas C.
Publication year - 1999
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.510290410
Subject(s) - comparative genomic hybridization , hepatocellular carcinoma , biology , gene , copy number variation , genetics , fluorescence in situ hybridization , genomic dna , cancer research , genome , chromosome
To search for recurrent and specific genomic alterations in human hepatocellular carcinoma (HCC), we examined 18 cell lines by comparative genomic hybridization (CGH), a molecular cytogenetic approach that allows positional identification of gains and losses of DNA sequences of the entire tumor genome. We report here a distinct pattern of multiple recurrent DNA copy‐number gains and losses that include alterations frequently seen in other neoplasias as well as changes potentially specific for HCC. The most frequent gains were localized on 1p34.3‐35, 1p33‐34.1, 1q21‐23, 1q31‐32, 6p11‐12, 7p21, 7q11.2, 8q24.1‐24.2, 11q11‐13, 12q11‐13, 12q23, 17q11.2‐21, 17q23‐24, and 20p11.1‐q13.2. Recurrent losses were mapped on 3p12‐14, 3q25, 4p12‐14, 4q13‐34, 5q21, 6q25‐26, 8p11.2‐23, 9p12‐24, 11q23‐24, 13q12‐33, 14q12‐13, 15q25‐26, 18q11.2‐22.2, and 21q21‐22. Seventeen genomic imbalances are novel in HCC, thus extending significantly the map of genetic changes and providing a starting point for the isolation of new genes relevant in pathogenesis of liver neoplasia, as well as providing molecular probes for both diagnosis and monitoring treatment of the disease