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Splenorenal shunt blood flow by transit‐time ultrasound as an index of collateral circulation in portal hypertensive rats
Author(s) -
Calès Paul,
Oberti Frédéric,
Veal Nary,
Fort Joël,
Kaassis Mehdi,
Moal Frédéric,
Aubé Christophe,
Vuillenin Eric,
Pilette Christophe,
Rifflet Hervé,
Trouvé Renaud
Publication year - 1998
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.510280515
Subject(s) - medicine , blood flow , hemodynamics , portal hypertension , ultrasound , shunt (medical) , collateral circulation , portosystemic shunt , cirrhosis , cardiology , nuclear medicine , radiology
The aim of this study was to develop a technique that could serve as an index of portosystemic shunt (PSS) blood flow in portal hypertensive rats whose main shunt is the splenorenal shunt (SRS). The main hemodynamic measurements performed were: SRS blood flow by the transit‐time ultrasound (TTU) method, percentage of PSS, and regional blood flows by the microsphere method. We determined the accuracy and reproducibility of SRS blood flow measurements under baseline and pharmacological (octreotide) conditions. SRS blood flow was compared with other hemodynamic characteristics. Two models of portal hypertension were used: secondary biliary and dimethylnitrosamine cirrhosis. The SRS blood flow was correlated with mesenteric ( r = .76; P < .001) and splenic ( r = .67; P < .01) PSS percentages. The intra‐ and interobserver agreements for SRS blood flow were excellent: r ic = .99 and r ic = .98, respectively. SRS blood flow was six times higher in portal hypertensive rats (0.6 ± 0.7 mL · min −1 · 100 g −1 ) than in sham rats (0.1 ± 0.1 mL · min −1 · 100 g −1 [ P < .01]). Octreotide significantly decreased SRS blood flow but not mesenteric or splenic PSS percentages. SRS is the main PSS in rats. The measurement of SRS blood flow by TTU is accurate and reproducible. This method can be used to identify new mechanisms in hemodynamic studies that differ from those identified by the measurement of the percentage of PSS by the microsphere method, especially in pharmacological studies.