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Hepatic regeneration in the isolated perfused rat liver followed by liver transplantation
Author(s) -
De Godoy José Luiz,
Fabre Monique,
Cherruau Brigitte,
McIntyre Maritza,
Soubrane Olivier,
Houssin Didier,
Cardoso Jorge E.
Publication year - 1998
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.510270310
Subject(s) - hepatocyte , hepatectomy , liver regeneration , transplantation , bolus (digestion) , perfusion , medicine , liver transplantation , endocrinology , andrology , biology , regeneration (biology) , surgery , in vitro , biochemistry , resection , microbiology and biotechnology
Controlling the S phase of the hepatocyte cell cycle would be of considerable help for stable retroviral foreign gene transfer. The aim of this article is to study hepatocyte regeneration during S phase in isolated, perfused rat liver followed by liver transplantation. Normal livers (G I: n = 7) were perfused with blood from normal rats for 6.1 ± 0.3 hours. Regenerating livers (G II; n = 7) obtained 18 hours after partial hepatectomy were perfused for 6.0 ± 0.3 hours with blood from rats partially hepatectomized 18 hours before. Regenerating livers (G III; n = 7) obtained 22 hours after partial hepatectomy were perfused for 2.4 ± 0.1 hours with blood from normal rats. In the normothermal perfusion system, a bolus of 25 mg of 5‐bromo‐2′‐deoxyuridine (BrdU) was added to the perfusate. Liver biopsies were taken at the end of each experiment. In group II, a biopsy was also taken 1 hour after BrdU introduction. At the end of each experiment, livers were orthotopically transplanted. The percentage of BrdU positive hepatocyte nuclei was 0.2% in G I; 14.8% and 38.4% after 1 hour and 6.1 hours, respectively, in G II; and 46.5% after 2.4 hours in G III. In G I, five rats died at day 1, 5, 6, 7, and 48 and two rats were still alive after 17 months. In G II, all the rats died before day five. In G III, two rats died at day one, one at day six, and four were still alive after 12 months. This study shows that, after 6 hours of normothermal perfusion, organ viability allows successful liver transplantation and that rat hepatocyte regeneration during cell cycle S phase in isolated normothermal conditions progresses in a similar way–quantity and timing–to liver regeneration found in vivo after partial hepatectomy.