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Quantitation of telomerase activity in hepatocellular carcinoma: A possible aid for a prediction of recurrent diseases in the remnant liver
Author(s) -
Suda Takeshi,
Isokawa Osamu,
Aoyagi Yutaka,
Nomoto Minoru,
Tsukada Kazuhiro,
Shimizu Takeaki,
Suzuki Yasufumi,
Naito Akira,
Igarashi Hirotaka,
Yanagi Masahiko,
Takahashi Toru,
Asakura Hitoshi
Publication year - 1998
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.510270213
Subject(s) - hepatocellular carcinoma , telomerase , medicine , oncology , carcinoma , cancer research , gastroenterology , pathology , biology , genetics , gene
Because telomerase activity is necessary for cell immortality and probably associated with tumor progression, we have evaluated a possible aid for quantitation of the activity to predict intrahepatic recurrences after surgery in patients with hepatocellular carcinoma (HCC). HCC tissues obtained by surgical resection from 20 patients were studied. Telomerase activity was expressed as peaks with a periodicity through a fluorescence‐based telomeric repeat amplification protocol using an autosequencer, and the quantity of activity was calculated from peak areas. A ratio of fluorescence intensity depending on telomerase to that of an internal standard was used as a value of relative telomerase activity (RTA). RTA in serially diluted S100 extracts from HepG2 cells was well correlated with the amount of the extracts. The mean RTA value of 36.4 ± 27.8 (mean ± SD, 3.21 to 105) in 9 patients suffering from early recurrences after surgery was significantly higher than that (9.84 ± 7.65; mean ± SD, 3.00 to 29.0) in 11 patients without intrahepatic recurrences during the early period ( P = .004). These results indicate that RTA value can be a useful predictor for intrahepatic recurrences during the early period after surgical resection of HCC.