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Bile acids suppress the secretion of very‐low‐density lipoprotein by human hepatocytes in primary culture
Author(s) -
Lin Y,
Havinga R,
Verkade H J,
Moshage H,
Slooff M J,
Vonk R J,
Kuipers F
Publication year - 1996
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.510230204
Subject(s) - secretion , primary culture , primary (astronomy) , chemistry , medicine , very low density lipoprotein , endocrinology , lipoprotein , biochemistry , microbiology and biotechnology , biology , cholesterol , in vitro , physics , astronomy
The existence of a relationship between bile acid and triacylglycerol metabolism in humans has been established, but the underlying mechanism and its physiological relevance have remained unclear. We have studied the effects of bile acids on the secretion of very‐low‐density lipoprotein (VLDL)‐associated triacylglycerol, using [ 3 H]glycerol labeling technique, and apolipoprotein B (apoB) in human hepatocytes in primary culture. Human hepatocytes secrete nascent VLDL with an average diameter of about 40 nm. Lipid composition of the particles resembles that reported for plasma VLDL, with the exception of a markedly lower cholesterylester content. In 24‐hour cultured human hepatocytes, physiological (i.e., portal) concentrations of taurocholic acid (10 to 200 μmol/L) suppressed [ 3 H]triacylglycerol secretion dose dependently. The degree of inhibition highly correlated ( r = .87, P < .01) with taurocholic acid content of the cells of different preparations (n = 7). ApoB secretion was inhibited by taurocholic acid to a similar extent as [ 3 H]triacylglycerol secretion ( r = .93, P < .01). Lipid composition of secreted VLDL particles did not change during taurocholic acid‐induced suppression. No effects on intracellular apoB, [ 3 H]triacylglycerol, triacylglycerol, and cholesterol mass were observed, nor did taurocholic acid affect protein synthesis, albumin secretion, or lactate dehydrogenase (LDH) release. Cellular cholesteryl ester (CHE) mass, however, was markedly reduced. Our results show that bile acids strongly interfere with the assembly or secretion of VLDL particles by human hepatocytes, suggesting a physiological function of the enterohepatic circulation of bile acids in the regulation of postprandial serum lipid levels.