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Intestinal Virome in Patients With Alcoholic Hepatitis
Author(s) -
Jiang Lu,
Lang Sonja,
Duan Yi,
Zhang Xinlian,
Gao Bei,
Chopyk Jessica,
Schwanemann Leila K.,
VenturaCots Meritxell,
Bataller Ramon,
BosquesPadilla Francisco,
Verna Elizabeth C.,
Abraldes Juan G.,
Brown Robert S.,
Vargas Victor,
Altamirano Jose,
Caballería Juan,
Shawcross Debbie L.,
Ho Samuel B.,
Louvet Alexandre,
Lucey Michael R.,
Mathurin Philippe,
GarciaTsao Guadalupe,
Kisseleva Tatiana,
Brenner David A.,
Tu Xin M.,
Stärkel Peter,
Pride David,
Fouts Derrick E.,
Schnabl Bernd
Publication year - 2020
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.31459
Subject(s) - human virome , feces , microbiome , biology , alcoholic liver disease , dysbiosis , virus , liver disease , alcoholic hepatitis , parvovirus , viral disease , hepatitis c virus , norovirus , torque teno virus , microbiology and biotechnology , virology , immunology , metagenomics , medicine , cirrhosis , polymerase chain reaction , genetics , gene , biochemistry
Background and Aims Alcoholic hepatitis (AH) is a severe manifestation of alcohol‐associated liver disease (ALD) with high mortality. Although gut bacteria and fungi modulate disease severity, little is known about the effects of the viral microbiome (virome) in patients with ALD. Approach and Results We extracted virus‐like particles from 89 patients with AH who were enrolled in a multicenter observational study, 36 with alcohol use disorder (AUD), and 17 persons without AUD (controls). Virus‐like particles from fecal samples were fractionated using differential filtration techniques, and metagenomic sequencing was performed to characterize intestinal viromes. We observed an increased viral diversity in fecal samples from patients with ALD, with the most significant changes in samples from patients with AH. Escherichia ‐, Enterobacteria ‐, and Enterococcus phages were over‐represented in fecal samples from patients with AH, along with significant increases in mammalian viruses such as Parvoviridae and Herpesviridae . Antibiotic treatment was associated with higher viral diversity. Specific viral taxa, such as Staphylococcus phages and Herpesviridae , were associated with increased disease severity, indicated by a higher median Model for End‐Stage Liver Disease score, and associated with increased 90‐day mortality. Conclusions In conclusion, intestinal viral taxa are altered in fecal samples from patients with AH and associated with disease severity and mortality. Our study describes an intestinal virome signature associated with AH.