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Retracted: M2 Macrophage–Derived Exosomes Facilitate HCC Metastasis by Transferring α M β 2 Integrin to Tumor Cells
Author(s) -
Wu Jindao,
Gao Wen,
Tang Qiyun,
Yu Yue,
You Wei,
Wu Zhengshan,
Fan Ye,
Zhang Long,
Wu Chen,
Han Guoyong,
Zuo Xueliang,
Zhang Yao,
Chen Zhiqiang,
Ding Wenzhou,
Li Xiangcheng,
Lin Fengming,
Shen Hongbing,
Tang Jinhai,
Zhang Yaqin,
Wang Xuehao
Publication year - 2021
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.31432
Subject(s) - microvesicles , cancer research , metastasis , integrin alpha m , tumor microenvironment , exosome , macrophage , tumor progression , integrin , chemistry , biology , cell , medicine , in vitro , immunology , cancer , microrna , tumor cells , immune system , biochemistry , gene
Background and Aims The development and progression of hepatocellular carcinoma (HCC) is dependent on its local microenvironment. Tumor‐associated macrophages (TAMs) are deemed a key factor for the tumor microenvironment and attribute to contribute to tumor aggressiveness. However, the detailed mechanism underlying the pro‐metastatic effect of TAMs on HCC remains undefined. Approach and Results The present study proved that TAMs were enriched in HCC. TAMs were characterized by an M2‐polarized phenotype and accelerated the migratory potential of HCC cells in vitro and in vivo . Furthermore, we found that M2‐derived exosomes induced TAM‐mediated pro‐migratory activity. With the use of mass spectrometry, we identified that integrin, α M β 2 (CD11b/CD18), was notably specific and efficient in M2 macrophage–derived exosomes (M2 exos). Blocking either CD11b and/or CD18 elicited a significant decrease in M2 exos–mediated HCC cell metastasis. Mechanistically, M2 exos mediated an intercellular transfer of the CD11b/CD18, activating the matrix metalloproteinase‐9 signaling pathway in recipient HCC cells to support tumor migration. Conclusions Collectively, the exosome‐mediated transfer of functional CD11b/CD18 protein from TAMs to tumor cells may have the potency to boost the migratory potential of HCC cells, thus providing insights into the mechanism of tumor metastasis.