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Theranostical nanosystem‐mediated identification of an oncogene and highly effective therapy in hepatocellular carcinoma
Author(s) -
Guo Yu,
Wang Jing,
Zhang Lu,
Shen Shunli,
Guo Ruomi,
Yang Yang,
Chen Wenjie,
Wang Yiru,
Chen Guihua,
Shuai Xintao
Publication year - 2016
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.28409
Subject(s) - hepatocellular carcinoma , cancer research , small interfering rna , oncogene , nanomedicine , wnt signaling pathway , angiogenesis , metastasis , medicine , genetic enhancement , in vivo , gene knockdown , cell culture , cancer , transfection , biology , signal transduction , gene , cell cycle , biochemistry , materials science , microbiology and biotechnology , nanoparticle , nanotechnology , genetics
Because the primary surgical treatment options for hepatocellular carcinoma (HCC)—including hepatic resection and liver transplantation—often fail due to recurrence and metastasis, identifying early prognostic biomarkers and therapeutic targets for HCC is of great importance. This study shows that transducin β‐like protein 1–related protein (TBLR1) is a key HCC oncogene that plays important roles in HCC proliferation, antiapoptosis, and angiogenesis by regulating the Wnt/β‐catenin pathway. The folate‐targeted theranostic small interfering RNA (siRNA) nanomedicine Fa‐PEG ‐g‐ PEI‐SPION/ p siRNA‐TBLR1 effectively silences the TBLR1 gene in different human HCC cell lines in vitro and in human HCC samples in vivo , resulting in the simultaneous suppression of HCC cell proliferation, antiapoptosis, and angiogenesis. Because of its multi‐anticancer functions against HCC, intravenous injection of the folate‐targeted siRNA nanomedicine into nude mice bearing intrahepatic or subcutaneous xenografts of human HCC has a significant therapeutic effect. Tumor growth in those animals was almost completely inhibited by treatment with Fa‐PEG ‐g‐ PEI‐SPION/ p siRNA‐TBLR1. Moreover, the SPION‐encapsulated polyplexes possess high magnetic resonance imaging (MRI) detection sensitivity, which makes tumor‐targeted siRNA delivery easily trackable using the clinical MRI technique. Conclusion : The theranostic siRNA nanomedicine examined here possesses great theranostic potential for combined gene therapy and MRI diagnosis of HCC. (H epatology 2016;63:1240–1255)