Premium
Mutant p53 in concert with an interleukin‐27 receptor alpha deficiency causes spontaneous liver inflammation, fibrosis, and steatosis in mice
Author(s) -
Dibra Denada,
Xia Xueqing,
Mitra Abhisek,
Cutrera Jeffry J.,
Lozano Guillermina,
Li Shulin
Publication year - 2016
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.28379
Subject(s) - steatosis , inflammation , fibrosis , nonalcoholic fatty liver disease , biology , hepatocyte , steatohepatitis , medicine , endocrinology , immunology , fatty liver , cancer research , disease , biochemistry , in vitro
The cellular and molecular etiology of unresolved chronic liver inflammation remains obscure. Whereas mutant p53 has gain‐of‐function properties in tumors, the role of this protein in liver inflammation is unknown. Herein, mutant p53 R172H is mechanistically linked to spontaneous and sustained liver inflammation and steatosis when combined with the absence of interleukin‐27 (IL27) signaling (IL27RA), resembling the phenotype observed in nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) patients. Indeed, these mice develop, with age, hepatocyte necrosis, immune cell infiltration, fibrosis, and micro‐ and macrosteatosis; however, these phenotypes are absent in mutant p53 R172H or IL27RA ‐/‐ mice. Mechanistically, endothelin A receptor (ETAR)‐positive macrophages are highly accumulated in the inflamed liver, and chemical inhibition of ETAR signaling reverses the observed phenotype and negatively regulates mutant p53 levels in macrophages. Conclusion: The combination of mutant p53 and IL27RA ‐/‐ causes spontaneous liver inflammation, steatosis, and fibrosis in vivo , whereas either gene alone in vivo has no effects on the liver. (H epatology 2016;63:1000–1012)