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Recruitment mechanisms of primary and malignant B cells to the human liver
Author(s) -
Shetty Shishir,
Bruns Tony,
Weston Christopher J.,
Stamataki Zania,
Oo Ye H.,
Long Heather M.,
Reynolds Gary M.,
Pratt Guy,
Moss Paul,
Jalkanen Sirpa,
Hubscher Stefan G.,
Lalor Patricia F.,
Adams David H.
Publication year - 2012
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.25790
Subject(s) - cell adhesion molecule , biology , cell adhesion , endothelial stem cell , pathology , integrin , endothelium , microbiology and biotechnology , chemokine , cancer research , receptor , immunology , cell , inflammation , medicine , endocrinology , in vitro , biochemistry , genetics
B cells are present within chronically inflamed liver tissue and recent evidence implicates them in the progression of liver disease. In addition, a large proportion of hepatic lymphomas are of B‐cell origin. The molecular signals that regulate normal and malignant B‐cell recruitment into peripheral tissue from blood are poorly understood, leading us to study human B‐cell migration through hepatic sinusoidal endothelial cells in flow‐based adhesion assays. In such assays, human blood‐derived B cells were captured from shear flow without a previous rolling phase and underwent firm adhesion mediated by vascular cell adhesion molecule‐1 (VCAM‐1). Unlike T cells, which displayed vigorous crawling behavior on the endothelium, B cells remained static before a proportion underwent transendothelial migration mediated by a combination of intercellular adhesion molecule‐1 (ICAM‐1), vascular adhesion protein‐1, common lymphatic endothelial and vascular endothelial receptor‐1/stabilin‐1, and the chemokine receptors, CXCR3 and CXCR4. B‐cell lymphoma cell lines and primary malignant B cells from patients with chronic lymphocytic leukemia and marginal zone B cell lymphoma also underwent integrin‐mediated firm adhesion involving ICAM‐1 and/or VCAM‐1 and demonstrated ICAM‐1‐dependent shape‐change and crawling behavior. Unlike primary lymphocytes, the malignant cells did not undergo transendothelial migration, which could explain why lymphomas are frequently characterized by the intravascular accumulation of malignant cells in the hepatic sinusoids. Conclusion: Our findings demonstrate that distinct combinations of signals promote B‐cell recruitment to the liver, suggesting the possibility of novel targets to modulate liver inflammation in disease. Certain features of lymphocyte homing are maintained in lymphoma recruitment to the liver, suggesting that therapeutic targets for lymphocyte recruitment may also prevent hepatic lymphoma dissemination. (H EPATOLOGY 2012)