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Hepatic stellate cells regulate immune response by way of induction of myeloid suppressor cells in mice
Author(s) -
Chou HongShiue,
Hsieh ChingChuan,
Yang HorngRen,
Wang Lianfu,
Arakawa Yusuke,
Brown Kathleen,
Wu Qingyu,
Lin Feng,
Peters Marion,
Fung John J.,
Lu Lina,
Qian Shiguang
Publication year - 2011
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.24162
Subject(s) - immune system , myeloid derived suppressor cell , hepatic stellate cell , stromal cell , immune tolerance , cancer research , immunology , bone marrow , biology , myeloid , microbiology and biotechnology , suppressor , cancer , endocrinology , genetics
Although organ transplants have been applied for decades, outcomes of somatic cell transplants remain disappointing, presumably due to lack of appropriate supporting stromal cells. Thus, cotransplantation with liver stromal cells, hepatic stellate cells (HSC), achieves long‐term survival of islet allografts in mice by way of induction of effector T cell apoptosis and generation of regulatory T (Treg) cells. In this study we provide evidence both in vitro and in vivo that HSC can promote generation of myeloid‐derived suppressor cells (MDSC). HSC‐induced MDSC demonstrate potent immune inhibitory activity. Induction of MDSC is dependent on an intact interferon gamma signaling pathway in HSC and is mediated by soluble factors, suggesting that the specific tissue stromal cells, such as HSC, play a crucial role in regulating immune response by way of inflammation‐induced generation of MDSC. Large amounts of MDSC can be propagated in vitro from bone marrow‐derived myeloid precursor cells under the influence of HSC. Conclusion: Cotransplantation with in vitro generated MDSC can effectively protect islet allografts from host immune attack. Local delivery of potent immune suppressor cells for cell transplants holds great clinical application potential. (H EPATOLOGY 2011;)