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MicroRNA‐125b suppressesed human liver cancer cell proliferation and metastasis by directly targeting oncogene LIN28B 2
Author(s) -
Liang Linhui,
Wong ChunMing,
Ying Qiao,
Fan Dorothy NgoYin,
Huang Shenglin,
Ding Jie,
Yao Jian,
Yan Mingxia,
Li Jinjun,
Yao Ming,
Ng Irene OiLin,
He Xianghuo
Publication year - 2010
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.23904
Subject(s) - oncogene , metastasis , microrna , cancer research , cell growth , cancer , medicine , oncology , biology , cell cycle , gene , genetics
MicroRNAs (miRNAs) are small, noncoding RNAs that can act as oncogenes or tumor suppressors in human cancer. Our previous study showed that miR‐125b was a prognostic indicator for patients with hepatocellular carcinoma (HCC), but its functions and exact mechanisms in hepatic carcinogenesis are still unknown. Here we demonstrate that miR‐125b suppressed HCC cell growth in vitro and in vivo . Moreover, miR‐125b increased p21Cip1/Waf1 expression and arrested cell cycle at G 1 to S transition. In addition, miR‐125b inhibited HCC cell migration and invasion. Further studies revealed that LIN28B was a downstream target of miR‐125b in HCC cells as miR‐125b bound directly to the 3′ untranslated region of LIN28B , thus reducing both the messenger RNA and protein levels of LIN28B . Silencing of LIN28B recapitulated the effects of miR‐125b overexpression, whereas enforced expression of LIN28B reversed the suppressive effects of miR‐125b. Conclusion: These findings indicate that miR‐125b exerts tumor‐suppressive effects in hepatic carcinogenesis through the suppression of oncogene LIN28B expression and suggest a therapeutic application of miR‐125b in HCC. (H EPATOLOGY 2010)