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Highly efficient generation of human hepatocyte–like cells from induced pluripotent stem cells
Author(s) -
SiTayeb Karim,
Noto Fallon K.,
Nagaoka Masato,
Li Jixuan,
Battle Michele A.,
Duris Christine,
North Paula E.,
Dalton Stephen,
Duncan Stephen A.
Publication year - 2010
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.23354
Subject(s) - induced pluripotent stem cell , hepatocyte , transplantation , stem cell , biology , economic shortage , microbiology and biotechnology , cancer research , embryonic stem cell , medicine , in vitro , biochemistry , gene , philosophy , linguistics , government (linguistics)
There exists a worldwide shortage of donor livers available for orthotropic liver transplantation and hepatocyte transplantation therapies. In addition to their therapeutic potential, primary human hepatocytes facilitate the study of molecular and genetic aspects of human hepatic disease and development and provide a platform for drug toxicity screens and identification of novel pharmaceuticals with potential to treat a wide array of metabolic diseases. The demand for human hepatocytes, therefore, heavily outweighs their availability. As an alternative to using donor livers as a source of primary hepatocytes, we explored the possibility of generating patient‐specific human hepatocytes from induced pluripotent stem (iPS) cells. Conclusion: We demonstrate that mouse iPS cells retain full potential for fetal liver development and describe a procedure that facilitates the efficient generation of highly differentiated human hepatocyte‐like cells from iPS cells that display key liver functions and can integrate into the hepatic parenchyma in vivo . (H EPATOLOGY 2010.)