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Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development
Author(s) -
Asahina Kinji,
Tsai Shirley Y.,
Li Peng,
Ishii Mamoru,
Maxson Robert E.,
Sucov Henry M.,
Tsukamoto Hidekazu
Publication year - 2009
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.22721
Subject(s) - mesenchymal stem cell , hepatic stellate cell , biology , alcam , microbiology and biotechnology , pathology , cell adhesion molecule , medicine , endocrinology
The knowledge concerning fetal hepatic stellate cells (HSCs) is scarce, and their cell lineage and functions are largely unknown. The current study isolated fetal liver mesenchymal cells from a mouse expressing β‐galactosidase under the control of Msx2 promoter by fluorescence‐activated cell sorting (FACS) and surveyed marker genes by microarray analysis. Based on the location and immunostaining with conventional and newly disclosed markers, we have identified three distinct populations of fetal liver mesenchymal cells expressing both desmin and p75 neurotrophin receptor (p75NTR): HSCs in the liver parenchyma; perivascular mesenchymal cells expressing α‐smooth muscle actin (α‐SMA); and submesothelial cells associated with the basal lamina beneath mesothelial cells and expressing activated leukocyte cell adhesion molecule (ALCAM) and platelet‐derived growth factor receptor α. A transitional cell type from the submesothelial cell phenotype to fetal HSCs was also identified near the liver surface. Mesothelial cells expressed podoplanin and ALCAM. Ki‐67 staining showed that proliferative activity of the submesothelial cells is higher than that of mesothelial cells and transitional cells. Using anti‐ALCAM antibodies, submesothelial and mesothelial cells were isolated by FACS. The ALCAM + cells expressed hepatocyte growth factor and pleiotrophin. In culture, the ALCAM + cells rapidly acquired myofibroblastic morphology and α‐SMA expression. The ALCAM + cells formed intracellular lipid droplets when embedded in collagen gel and treated with retinol, suggesting the potential for ALCAM + cells to differentiate to HSCs. Finally, we demonstrated that fetal HSCs, submesothelial cells, and perivascular mesenchymal cells are all derived from mesoderm by using MesP1‐Cre and ROSA26 reporter mice. Conclusion: Fetal HSCs, submesothelial cells, and perivascular mesenchymal cells are mesodermal in origin, and ALCAM + submesothelial cells may be a precursor for HSCs in developing liver. (H EPATOLOGY 2009.)