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Palmitic acid induces production of proinflammatory cytokine interleukin‐8 from hepatocytes
Author(s) -
JoshiBarve Swati,
Barve Shirish S.,
Amancherla Kiranmayi,
Gobejishvili Leila,
Hill Daniell,
Cave Matthew,
Hote Prachi,
McClain Craig J.
Publication year - 2007
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.21752
Subject(s) - proinflammatory cytokine , steatosis , nonalcoholic fatty liver disease , endocrinology , medicine , inflammation , palmitic acid , fatty liver , biology , chemistry , fatty acid , biochemistry , disease
Obesity and the metabolic syndrome are closely correlated with hepatic steatosis. Simple hepatic steatosis in nonalcoholic fatty liver disease can progress to nonalcoholic steatohepatitis (NASH), which can be a precursor to more serious liver diseases, such as cirrhosis and hepatocellular carcinoma. The pathogenic mechanisms underlying progression of steatosis to NASH remain unclear; however, inflammation, proinflammatory cytokines, and oxidative stress have been postulated to play key roles. We previously reported that patients with NASH have elevated serum levels of proinflammatory cytokines, such as interleukin‐8 (IL‐8), which are likely to contribute to hepatic injury. This study specifically examines the effect of hepatic steatosis on IL‐8 production. We induced lipid accumulation in hepatocytes (HepG2, rat primary hepatocytes, and human primary hepatocytes) by exposing them to pathophysiologically relevant concentrations of palmitic acid to simulate the excessive influx of fatty acids into hepatocytes. Significant fat accumulation was documented morphologically by Oil Red O staining in cells exposed to palmitic acid, and it was accompanied by an increase in intracellular triglyceride levels. Importantly, palmitic acid was found to induce significantly elevated levels of biologically active neutrophil chemoattractant, IL‐8, from steatotic hepatocytes. Incubation of the cells with palmitate led to increased IL‐8 gene expression and secretion (both mRNA and protein) through mechanisms involving activation of nuclear factor kappaB (NF‐κB) and c‐Jun N‐terminal kinase/activator protein‐1. Conclusion: These data demonstrate for the first time that lipid accumulation in hepatocytes can stimulate IL‐8 production, thereby potentially contributing to hepatic inflammation and consequent liver injury. (H EPATOLOGY 2007.)