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A picture says more than a thousand words: Structural insights into hepatitis C virus translation initiation
Author(s) -
Bellecave Pantxika,
Moradpour Darius
Publication year - 2006
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.21450
Subject(s) - internal ribosome entry site , eukaryotic small ribosomal subunit , biology , initiation factor , eukaryotic initiation factor , microbiology and biotechnology , eukaryotic translation , translation (biology) , ribosome , messenger rna , ribosomal rna , eukaryotic ribosome , protein subunit , rna , genetics , gene
Protein synthesis in mammalian cells requires initiation factor eIF3, a ∼750‐kilodalton complex that controls assembly of 40S ribosomal subunits on messenger RNAs (mRNAs) bearing either a 5′‐cap or an internal ribosome entry site (IRES). Cryoelectron microscopy reconstructions show that eIF3, a five‐lobed particle, interacts with the hepatitis C virus (HCV) IRES RNA and the 5′‐cap binding complex eIF4F via the same domain. Detailed modeling of eIF3 and eIF4F onto the 40S ribosomal subunit reveals that eIF3 uses eIF4F or the HCV IRES in structurally similar ways to position the mRNA strand near the exit site of 40S, promoting initiation complex assembly.