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Troglitazone inhibits tumor growth in hepatocellular carcinoma in vitro and in vivo
Author(s) -
Yu Jun,
Qiao Liang,
Zimmermann Lars,
Ebert Matthias P. A.,
Zhang Hongxia,
Lin Wendy,
Röcken Christoph,
Malfertheiner Peter,
Farrell Geoffrey C.
Publication year - 2006
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.20994
Subject(s) - troglitazone , apoptosis , cell growth , cell cycle , peroxisome proliferator activated receptor , proliferating cell nuclear antigen , cancer research , endocrinology , biology , medicine , tunel assay , cell culture , in vivo , receptor , biochemistry , genetics , microbiology and biotechnology
Abstract Peroxisome proliferator‐activated receptor γ (PPARγ) has been implicated in the differentiation and growth inhibition of cancer cells. We examined the effects of PPARγ activation by troglitazone on hepatocellular carcinoma (HCC) cell growth, proliferation, and apoptosis in vitro and in vivo . We also studied relationships between PPARγ activation and cyclooxygenase‐2 (COX‐2) expression. Human HCC cell lines Huh7 and Hep3B were cultured in the presence or absence of troglitazone. Cell growth was determined via WST‐1 assay, proliferation by cell cycle analysis and proliferating cell nuclear antigen (PCNA) Western blotting, and apoptosis by flow cytometry and TUNEL. Tumor growth after subcutaneous implantation of Huh7 cells in nude mice was monitored, and the effects of treatment with troglitazone were determined. In resected HCCs, PPARγ expression was less compared with the histologically normal surrounding liver. In cultures of Hep3B and Huh7 cells, basal expression of PPARγ was relatively low, but troglitazone caused dose‐dependent induction of PPARγ expression. Cell cycle analysis revealed a decreased proportion of cells in S phase, with arrest at G0/G1. Concomitant downregulation of PCNA and an increase in TUNEL staining, cells were consistent with decreased proliferation and induction of apoptosis by troglitazaone. Troglitazone‐mediated PPARγ activation also suppressed COX‐2 expression and induced p27 in HCC cells. Administration of troglitazone to Huh7 tumor‐bearing mice significantly reduced tumor growth and caused tumor regression. In conclusion , collectively, these results indicate that PPARγ could be a regulator of cell survival and growth in HCC. PPARγ therefore represents a putative molecular target for chemopreventive therapy or inhibition of liver cancer growth.. (H EPATOLOGY 2006;43:134–143.)