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Treating hepatitis C: Can you teach old dogs new tricks?
Author(s) -
Rice Charles M.,
You Shihyun
Publication year - 2005
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.20975
Subject(s) - ns5b , biology , viral replication , hepatitis c virus , virology , rna dependent rna polymerase , rna polymerase , rna , rna interference , replication (statistics) , genetics , virus , hepacivirus , gene
Viruses depend on host‐derived factors for their efficient genome replication. Here, we demonstrate that a cellular peptidyl‐prolyl cis‐trans isomerase (PPIase), cyclophilin B (CyPB), is critical for the efficient replication of the hepatitis C virus genome. CyPB interacted with the HCV RNA polymerase NS5B to directly stimulate its RNA binding activity. Both the RNA interference (RNAi)‐mediated reduction of endogenous CyPB expression and the induced loss of NS5B binding to CyPB decreased the levels of HCV replication. Thus, CyPB functions as a stimulatory regulator of NS5B in HCV replication machinery. This regulation mechanism for viral replication identifies CyPB as a target for antiviral therapeutic strategies.