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Down‐regulation of the organic cation transporter 1 of rat liver in obstructive cholestasis
Author(s) -
Denk Gerald U.,
Soroka Carol J.,
Men Albert,
Koepsell Hermann,
Beuers Ulrich,
Boyer James L.
Publication year - 2004
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.20176
Subject(s) - cholestasis , organic anion transporter 1 , organic cation transport proteins , kidney , transporter , medicine , endocrinology , tetraethylammonium , reabsorption , messenger rna , chemistry , hepatocyte , multidrug resistance associated protein 2 , biology , biochemistry , atp binding cassette transporter , gene , potassium , organic chemistry , in vitro
The liver plays a major role in biotransformation and elimination of various therapeutic agents and xenobiotics, many of which are organic cations and substrates of the organic cation transporter 1 (Oct1, Slc22a1). Oct1 is expressed at the basolateral membranes of hepatocytes and proximal renal tubules. Although Oct1 is the major uptake mechanism in hepatocytes for many pharmaceutical compounds, little is known about the effects of liver injury on this process. Our aim was to investigate the effects of obstructive cholestasis on Oct1 expression and function in liver and kidney. The effects of bile duct ligation (BDL) on Oct1 protein, messenger RNA (mRNA) expression, and tissue localization were determined in rat liver and kidney with Western analysis, real‐time reverse transcriptase‐mediated polymerase chain reaction (RT‐PCR), and immunofluorescence. To assess Oct1 function, the model substrate tetraethylammonium ([ 14 C]TEA) was administered intravenously to BDL and control rats and distribution of radioactivity was determined. Oct1 protein significantly decreased in cholestatic livers to 42.1 ± 17.7% ( P < .001), 15.5 ± 4.7% ( P < .05), and 8.6 ± 2.7% ( P < .05) of controls after 3, 7, and 14 days, respectively, but not in kidneys. Hepatic Oct1 mRNA decreased to 77.2 ± 12.7%, 40.7 ± 8.1% ( P < .05), and 50.3 ± 7.5% ( P < .05) 3, 7, and 14 days after BDL, respectively. Tissue immunofluorescence corroborated these data. Hepatic accumulation of [ 14 C]TEA in 14‐day BDL rats was reduced to 29.6 ± 10.9% of controls ( P < .0005). In conclusion, obstructive cholestasis down‐regulates Oct1 and impairs Oct1‐mediated uptake in rat liver, suggesting that hepatic uptake of small cationic drugs may be impaired in cholestatic liver injury. (H EPATOLOGY 2004;39:1382–1389.)