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Ischemic preconditioning affects interleukin release in fatty livers of rats undergoing ischemia/reperfusion
Author(s) -
Serafín Anna,
RosellóCatafau Joan,
Prats Neus,
Gelpí Emilio,
Rodés Joan,
Peralta Carmen
Publication year - 2004
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.20089
Subject(s) - ischemic preconditioning , medicine , ischemia , nitric oxide , interleukin , reperfusion injury , receptor antagonist , interleukin 10 , interleukin 1β , pharmacology , endocrinology , antagonist , cytokine , receptor
The present study evaluates the effect of ischemic preconditioning on interleukin‐1 (IL‐1) and interleukin‐10 (IL‐10) generation following hepatic ischemia/reperfusion (I/R) in normal and steatotic livers as well as the role of nitric oxide (NO) in this process. Increased IL‐1β and IL‐10 levels were observed in normal livers after I/R. Steatotic livers showed higher IL‐1β levels than normal livers, and IL‐10 at control levels. The injurious role of IL‐1β and the benefits of IL‐10 on hepatic I/R injury was shown with the use of IL‐1 receptor antagonist (IL‐1ra), anti‐IL‐10 polyclonal antibody against IL‐10 (anti‐IL‐10) and exogenous IL‐10. The effective dose of these treatments was different in both types of livers. Preconditioning prevented IL‐1β release and increased IL‐10 generation after I/R in normal and steatotic livers. IL‐1β or anti‐IL‐10 pretreatments reversed the benefits of preconditioning. IL‐1β action inhibition in a preconditioned group that was pretreated with anti‐IL‐10 did not modify the benefits of preconditioning. In addition, anti‐IL‐10 pretreatment in the preconditioned group resulted in IL‐1β levels comparable to those observed after I/R. NO inhibition eliminated the benefits of preconditioning on IL‐10 release, IL‐1β levels, and hepatic injury. In conclusion, preconditioning, through IL‐10 overproduction, inhibits IL‐1β release and the ensuing hepatic I/R injury in normal and steatotic livers. IL‐10 generation induced by preconditioning could be mediated by NO. (H EPATOLOGY 2004;39:688–698.)