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Differential expression of A and B laminin chains during rat liver regeneration
Author(s) -
Giménez América,
Hostench Joaquim,
Stamatoglou Stamatis C.,
Enrich Carlos
Publication year - 1995
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840220435
Subject(s) - laminin , regeneration (biology) , liver regeneration , differential (mechanical device) , microbiology and biotechnology , chemistry , biology , physics , extracellular matrix , thermodynamics
We have studied the distribution and expression of laminin during rat liver regeneration by immunofluorescence and immunoblotting using affinity‐purified laminin antibodies. Laminin was localized on sinusoidal surfaces in normal and regenerating hepatic parenchyma, but enhanced expression was detected during regeneration from 6 hours to 7 days after a partial hepatectomy. A and B laminin chains were specifically detected by Western blotting in highly purified plasma membrane fractions derived from the sinusoidal domain of hepatocytes. Analysis of laminin expression in whole homogenates from hepatectomized rats showed a differential expression of A and B laminin chains during regeneration: The A chain showed a progressive increase, whereas the B chain exhibited an early, prereplicative increase and then gradually declined to control levels at the end of restorative growth. The results suggest differential temporal requirements for A and B laminin chains during hepatic growth and tissue formation. (H EPATOLOGY 1995; 22:1259–1262.).