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Anti‐hepatitis B virus activity of a mixture of two monoclonal antibodies in an “inhibition in solution” assay
Author(s) -
Heijtink Rudolf A.,
Kruining Johannes,
Weber Yvonne A. M.,
de Man Robert A.,
Schalm Solko W.
Publication year - 1995
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840220410
Subject(s) - hbsag , monoclonal antibody , epitope , virology , antibody , hepatitis b virus , microbiology and biotechnology , monoclonal , hepatology , antigen , medicine , hepatitis c virus , hepatitis b , virus , immunology , biology
Two human monoclonal antibodies with anti‐hepatitis B activity were investigated separately and as a mixture by means of an “inhibition in solution” assay. With this assay the capacity of anti‐HBs antibodies to inhibit the binding of hepatitis B surface antigen (HBsAg) with solid‐phase anti‐HBs (Ausria II, Abbott Laboratories) was studied. Both HBsAg of different subtypes and purified Dane particles were used. One of the monoclonal antibodies (9H9) was directed against a conformational epitope (anti‐“a” like) but induced incomplete inhibition (80% to 90%) of all HBsAg subtypes; the other (4–7B), active against a linear epitope, caused full inhibition of all HBsAg subtypes except one (HBsAg/adw 4 ). In vitro , a mixture of these two monoclonal antibodies was active against HBsAg from liver transplant recipients, including those with suspected variant viruses. (H EPATOLOGY 1995; 22:1078–1083.).