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Effects of extracellular matrices on tube formation of cultured rat hepatic sinusoidal endothelial cells
Author(s) -
Shakado Satoshi,
Sakisaka Shotaro,
Noguchi Kazunori,
Yoshitake Masao,
Harada Masaru,
Mimura Yoshihiro,
Sata Michio,
Tanikawa Kyuichi
Publication year - 1995
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840220339
Subject(s) - matrigel , laminin , extracellular matrix , endothelial stem cell , hepatic stellate cell , type i collagen , type iv collagen , extracellular , biology , chemistry , microbiology and biotechnology , in vitro , biochemistry , endocrinology
To determine the effects of extracellular matrices on the function and morphology of hepatic sinusoidal endothelial cells, isolated rat hepatic sinusoidal endothelial cells were cultured in three‐dimensional fashion on collagen gel containing various extracellular matrix components. Cells cultured on type I collagen gel with or without type IV collagen formed a cobblestone appearance on the surface of the gel. Cells cultured on laminincontaining type I collagen gel invaded the gel and exhibited three‐dimensional tube formation with a decreased number of characteristic endothelial pores. Morphometrically, there was a significant relationship between the length of the tube formed and the concentration of laminin in the type I collagen gel. Cells cultured on Matrigel, which contains high concentrations of laminin, type IV collagen, fibroblast growth factor, tissue plasminogen activator, and other growth factors, formed a great number of tubes into a network on the surface of the gel, as is observed in in situ hepatic sinusoidal endothelial cells. Ultrastructurally, tube‐forming endothelial cells cultured on Matrigel had many endothelial pores on the cell surface, with tubes (approximately 10 μm in diameter) formed by two or three hepatic sinusoidal endothelial cells. These results indicated that extracellular matrix components, especially laminin, induced the formation of tubes in cultured rat hepatic sinusoidal endothelial cells. Tube‐forming sinusoidal endothelial cells cultured on Matrigel could provide more advantages than the two‐dimensional culture model for investigating the function and morphology of these cells in vitro. (Hepatology 1995; 22:969–973.)