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Transport proteins and molecular biology: Is cloning the beginning or the end?
Author(s) -
Stump Decherd,
Weisiger Richard A.,
Berk Paul D.
Publication year - 1995
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840220244
Subject(s) - cloning (programming) , computational biology , biology , microbiology and biotechnology , computer science , programming language
Long chain fatty acids (LCFAs) are an important energy substrate used by cardiac myocytes and other cells, but the mechanism whereby these molecules cross the plasma membrane is poorly understood. We used an expression cloning strategy and a cDNA library from 3T3‐L1 adipocytes to identify a cDNA that, when expressed in cultured cells, augments uptake of LCFAs. This cDNA encodes a novel 646 amino acid fatty acid transport protein (FATP) with six predicted membrane spanning regions and that is integrally associated with membranes. Immunocytochemistry and subcellular fractionation of 3T3‐L1 adipocytes show that FATP is localized to the plasma membrane. We propose that FATP is a plasma membrane transporter for LCFAs.