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Association of primary biliary cirrhosis with the allele HLA‐DPB1*0301 in a German population
Author(s) -
Mella Juan G.,
Roschmann Elke,
Maier KlausPeter,
Volk Brigitte A.
Publication year - 1995
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840210221
Subject(s) - primary biliary cirrhosis , allele , german population , human leukocyte antigen , medicine , german , population , immunology , gastroenterology , genetics , biology , antigen , philosophy , environmental health , gene , linguistics
The major histocompatibility complex class II alleles at the HLA‐DPB1 locus were investigated in 32 German Caucasoid patients with primary biliary cirrhosis (PBC) and compared with those from 47 normal control patients using molecular genotyping techniques. The second exon of the HLA‐DPB1 gene was amplified by polymerase chain reaction (PCR) and hybridized with 25 sequence‐specific oligonucleotides (SSOs) to assign the HLA‐DPB1 alleles on the basis of known sequence variations, according to the protocols of the Eleventh International Histocompatibility Workshop. A strong association of PBC was found with the allele HLA‐DPB1*0301. The allele HLA DPB1*0301 was present in 50% (16 of 32) of the patients with PBC compared with 13% (6 of 47) or normal controls ( P corrected < .015), whereas the other HLA‐DPB1 alleles showed no significant differences in both groups. The relative risk (RR) estimate for the allele HLA‐DPB1*0301 was 6.8 (95% confidence limits: 2.27 to 20.57). In summary, this study clearly demonstrates an association of PBC with the HLA‐DPB1*0301 allele in German Caucasoids and may add new data to the immunogenetic background of PBC, suggesting a contribution of the HLA‐DPB1 gene to the genetic susceptibility of the disease. (H EPATOLOGY 1995;21:398–402.)