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Identification of the thromboxane A 2 receptor in hepatic sinusoidal endothelial cells and its role in Endotoxin‐induced liver injury in rats
Author(s) -
Ishiguro Satoshi,
Arii Shigeki,
Monden Kazunobu,
Adachi Yukito,
Funaki Naomi,
Higashitsuji Hiroaki,
Fujita ShinIchi,
Furutani Masaharu,
Mise Masahiro,
Kitao Tadahiro,
Nakamura Toshio,
Ushikubi Fumitaka,
Nakamura KenIchi,
Narumiya Shuh,
Enomoto Katsuhiko,
Ohmura Tousei,
Mori Michio,
Imamura Masayuki
Publication year - 1994
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840200527
Subject(s) - thromboxane a2 , cyclooxygenase , receptor , thromboxane , agonist , dissociation constant , binding site , medicine , endocrinology , endothelial stem cell , chemistry , biology , biochemistry , enzyme , platelet , in vitro
Abstract The presence of the thromboxane A 2 receptor in sinusoidal endothelial cells was investigated and its pathogenic role in endotoxin‐induced liver injury examined. The receptor was measured with a binding assay using a specific thromboxane A 2 receptor antagonist, [ 3 H]S‐145. Scatchard analysis of the binding indicated the presence of a single class of high‐affinity binding sites with a dissociation constant of 5.00 ± 0.96 nmol/L, a maximal binding of 22.85 ± 2.71 fmol/10 6 cells and 13.80 ± 1.60 × 10 3 binding sites per cell. The addition of a cyclooxygenase inhibitor, indomethacin, during the cell preparation increased the maximal binding value and the number of binding sites of 37.34 ± 3.01 and 22.50 ± 1.80 × 10 3 sites/cell, respectively. The binding was displaced by various thromboxane A 2 analogs such as ONO‐3708 and STA 2 but was not effectively competed for by other prostaglandins. Endotoxin injection reduced dissociation constant, maximal binding and the number of binding sites in sinusoidal endothelial cells to 3.49 ± 0.87 nmol/L, 6.03 ± 0.64 fmol/10 6 cells and 3.65 ± 0.39 × 10 3 sites/cell, respectively. A cyclooxygenase inhibitor and a Kupffer cell inhibitor added before endotoxin treatment significantly prevented the reduction in the number of thromboxane A 2 receptors. It is possible that these effects were due to a reduction in the agonist‐induced internalization of the thromboxane A 2 receptor brought about by the prevention of thromboxane A 2 production. Preadministration of both a cyclooxygenase inhibitor and a thromboxane A 2 receptor antagonist attenuated the degree of endotoxininduced liver injury. These findings indicated that the thromboxane A 2 —thromboxane A 2 receptor system in the hepatic sinusoids plays a significant role in the pathogenesis of endotoxin‐dependent liver damage. (Hepatology 1994;20:1281–1286).