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Impaired ability of neutrophils to produce oxygen‐derived free radicals in patients with chronic liver disease and hepatocellular carcinoma
Author(s) -
Uehara Masahiro,
Sato Noriyuki
Publication year - 1994
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840200209
Subject(s) - chemiluminescence , luminol , chemistry , luciferin , cirrhosis , hepatocellular carcinoma , reactive oxygen species , biochemistry , photochemistry , medicine , luciferase , chromatography , transfection , gene
Abstract To evaluate whether neutrophil bactericidal function, the ability to produce oxygen‐derived free radicals, is altered in patients with chronic liver disease, we measured chemiluminescence amplified by a luciferin analog (Cypridina luciferin analog‐dependent chemiluminescence) and luminol (luminol‐dependent chemiluminescence) in response to N‐formyl‐Met‐Lue‐Phe by neutrophils from patients with chronic liver diseases due to C and/or B type hepatitis: chronic active hepatitis, cirrhosis and hepatocellular carcinoma. Both Cypridina luciferin analog‐dependent chemiluminescence and luminol‐dependent chemiluminescence were significantly decreased in neutrophils from patients with chronic liver disease (hepatocellular carcinoma