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Diagnostic and clinical implications of the different genotypes of hepatitis C virus
Author(s) -
Bukh Jens,
Miller Roger H.
Publication year - 1994
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840200139
Subject(s) - genotype , virology , hepatitis c virus , biology , recombinant dna , antibody , polymerase chain reaction , virus , hepacivirus , gene , microbiology and biotechnology , immunology , genetics
Hepatitis C virus (HCV) samples in 155 sera, from patients with chronic non‐A, non‐B liver disease and blood donors, were grouped into four genotypes (I, II, III, and IV) by amplification of core‐gene sequences by polymerase chain reaction with type‐specific primers. HCV genotypes were compared with various HCV‐associated antibodies detectable by the first‐generation ELISA (ELISA‐1) with C100‐3 protein and a second‐generation immunoblot assay with four recombinant HCV proteins. Antibodies to C100‐3 protein and those to its subsequence (5–1–1) were detected in 13 (93%) and 12 (86%), respectively, of 14 sera with genotype I HCV; 56 (79%) and 58 (82%) of 71 sera with genotype II; 13 (34%) and 6 (16%) of 38 sera with genotype III; and 11 (34%) and 4 (13%) of 32 sera with genotype IV. Amino acid sequences of C100‐3 of genotype I HCV are conserved by ∼90% in genotype II, but only by ∼75% in genotypes III and IV. The sensitivity of ELSA‐1, therefore, would be influenced by heterogeneity in C100‐3 sequences of different genotypes.