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Hepatic biliary transport after hepatocyte transplantation in eizai hyperbilirubinemic rats
Author(s) -
Hamaguchi Hiromitsu,
Yamaguchi Yasuo,
Goto Mataro,
Misumi Mikio,
Hisama Naoya,
Miyanari Nobutomo,
Mori Katsutaka,
Ogawa Michio
Publication year - 1994
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840200132
Subject(s) - medicine , hepatocyte , bilirubin , endocrinology , hepatectomy , hepatology , excretory system , sulfobromophthalein , liver transplantation , transplantation , chemistry , liver function tests , biochemistry , in vitro , surgery , resection
The Eizai hyperbilirubinemic rat (EHBR) is a Sprague‐Dawley mutant rat with conjugated hyperbilirubinemia. Eizai hyperbilirubinemic rats have impaired canalicular excretory transport of organic anions, bile acid glucuronide and sulfate. Eizai hyperbilirubinemic rats, with and without a 68% partial hepatectomy, were treated by an intraportal injection of 1×10 7 wild‐type Sprague‐Dawley mutant rat hepatocytes. Serum bilirubin concentrations decreased significantly after hepatectomy and intraportal injection. Biliary excretion was demonstrated by biliary transport of indocyanine and sulfobromophthalein into the bile. These results indicate that hepatic transport of bile acid conjugates in Eizai hyperbilirubinemic rats can be restored by hepatocyte transplantation in conjunction with the recipient's excretory biliary system. (Hepatology 1994;20:220‐224.)