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Proliferation of rat liver macrophages in vitro : Influence of hemopoietic growth factors
Author(s) -
Hoedemakers Rein M. J.,
Scherphof Gerrit L.,
Daemen Toos
Publication year - 1994
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840190318
Subject(s) - macrophage , haematopoiesis , colony stimulating factor , granulocyte macrophage colony stimulating factor , biology , in vitro , granulocyte macrophage colony stimulating factor receptor , hematopoietic growth factor , cytokine , macrophage colony stimulating factor , growth factor , granulocyte , lymphokine , recombinant dna , thymidine , tumor necrosis factor alpha , cell culture , interleukin 3 , immunology , biochemistry , microbiology and biotechnology , stem cell , receptor , cytotoxic t cell , antigen presenting cell , gene , genetics
We examined the effects of several hemopoietic growth factors on proliferation of rat liver macrophages in vitro. The proliferative response of liver macrophages to hemopoietic growth factors was assayed on the basis of [methyl‐ 3 H]thymidine uptake. Macrophage colony‐stimulating factor and recombinant murine granulocyte‐macrophage colony‐stimulating factor stimulated [methyl‐ 3 H]thymidine incorporation in a concentration‐dependent manner. With granulocyte‐macrophage colony‐stimulating factor, maximum incorporation was observed at 50 U/ml, whereas with macrophage colony‐stimulating factor no incorporation plateau was observed up to 50 L929‐conditioned medium. Incubation of liver macrophages with various concentrations of recombinant human interleukin‐2, recombinant murine interleukin‐3 and recombinant human interleukin‐6 or culture medium alone did not result in significant incorporation of [methyl‐ 3 H]thymidine. When liver macrophages were fractionated according to cell size, highest incorporation was observed in the large macrophages. Proliferating cells in cultures of all subfractions were microscopically identified as typical macrophages by the use of macrophage‐specific monoclonal antibodies. After 6 days in culture, these macrophages had functional properties similar to those of resident liver macrophages with respect to phagocytosis and in vitro activation with immunomodulators to tumorcytotoxicity and secretion of nitric oxide and tumor necrosis factor‐α. These results suggest that macrophage colony‐stimulating factor and granulocyte‐macrophage colony‐stimulating factor play important roles among the regulatory factors that support local proliferation of rat liver macrophages. (Hepatology 1994;19:666–674).