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Serum levels of transforming growth factor‐α in patients after partial hepatectomy as determined with an enzyme‐linked immunosorbent assay
Author(s) -
Tomiya Tomoaki,
Fujiwara Kenji
Publication year - 1993
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840180213
Subject(s) - transforming growth factor , hepatectomy , medicine , polyclonal antibodies , hepatology , liver regeneration , endocrinology , growth factor , antibody , biology , immunology , resection , surgery , regeneration (biology) , receptor , microbiology and biotechnology
A sandwich enzyme‐linked immunosorbent assay for measuring serum transforming growth factor‐α levels was developed with monoclonal IgM and polyclonal IgG antihuman transforming growth factor‐α antibodies and a system to amplify the activity of the conjugated enzyme. The assay detected serum transforming growth factor‐α levels as low as 5 pg/ml. Serum transforming growth factor‐α levels were below the detection limit of the assay in 19% of healthy adults, with a mean (± S.D.) detectable level of 22.0 ± 16.7 pg/ml. In 13 patients who underwent partial hepatectomy, serum transforming growth factor‐α levels were determined serially after surgery. The levels were increased within 28 days of surgery in all the patients, with a mean maximal level of 118.2 ± 90.8 pg/ml. The maximal level achieved in each case correlated significantly with the resected volume of the liver and the increased volume of the remaining liver 28 days after hepatectomy (r = 0.59, p < 0.05, and r = 0.71, p < 0.005, respectively). In contrast, serum transforming growth factor‐α levels showed no increase after laparotomy for nonhepatic surgery. Serum transforming growth factor‐α levels may vary depending on the regenerative stimulus and reflect the degree to which liver regeneration will occur in patients after partial hepatectomy. (H EPATOLOGY 1993;18:304–308).