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Association of primary biliary cirrhosis with human leukocyte antigen DPB1*0501 in Japanese patients
Author(s) -
Seki Takeshi,
Kiyosawa Kendo,
Ota Masao,
Furuta Seiichi,
Fukushima Hirofumi,
Tanaka Eiji,
Yoshizawa Kaname,
Kumagai Toshiko,
Mizuki Nobuhisa,
And Asako,
Inok Hidetoshi
Publication year - 1993
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840180113
Subject(s) - primary biliary cirrhosis , medicine , biliary cirrhosis , antigen , human leukocyte antigen , gastroenterology , immunology , antibody , autoimmune disease
To investigate the relationship between distribution of human leukocyte antigen alleles and susceptibility to primary biliary cirrhosis among Japanese, we performed serological typing and human leukocyte antigen DP genotyping in 47 Japanese patients with primary biliary cirrhosis. Serologically, the frequency of human leukocyte antigen DQ3 was significantly higher in the patients than in healthy controls, whereas frequency of DR52 was significantly lower in the patients. Human leukocyte antigen DP genotyping using the polymerase chain reaction–restriction‐fragment‐length polymorphism method showed that the frequency of human leukocyte antigen DPB1*0501 (85.1%) was significantly higher in patients than in healthy controls but that the frequency of DPB1*0402 was significantly lower in patients. The positive association of human leukocyte antigen DPB1*0501 with Primary biliary cirrhosis was stronger than that of serological human leukocyte antigen DQ3, which can be explained by its linkage to DPB1*0501. In addition, three of seven DPB1*0501‐negative patients had DPB1*0202, suggesting that the human leukocyte antigen DPB1 amino‐acid chain plays an important role in the immunogenesis of primary biliary cirrhosis among Japanese patients. Comparative analysis of amino acid sequences from DPB1 alleles indicated that a Leu at position 35 of the DPB1 chain likely contributes to the susceptibility to primary biliary cirrhosis among the Japanese. (H EPATOLOGY 1993;18:73–78).