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Can the liver be induced to say no?
Author(s) -
Wiley John W.
Publication year - 1993
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840170634
Subject(s) - nitric oxide , incubation , nitric oxide synthase , lipopolysaccharide , protein kinase c , stimulation , phorbol , chemistry , incubation period , biochemistry , biology , endocrinology , phosphorylation
The incubation of primary cultures of rat hepatocytes with lipopolysaccharide (LPS) or biologically active phorbol esters promotes the release of nitric oxide to the incubation medium. This process is the result of the induction of the Ca 2+ ‐ and calmodulinindependent form of nitric oxide synthase. Both the release of nitric oxide to the incubation medium and the expression of nitric oxide synthase activity exhibited a lag period of about 45–60 min after cell stimulation. Exposure of hepatocytes to both stimuli produced an antagonistic effect on nitric oxide release, with a half‐maximal inhibition obtained with 14 nM phorbol 12,13‐dibutyrate at saturating concentration of LPS. Incubation of cells with α‐phorbol 12,13‐ didecanoate failed to counteract the effect of LPS or to induce nitric oxide synthase, suggesting that activation of protein kinase C was involved in this process.