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Cholesterol cholelithiasis in the prairie dog: Role of mucin and nonmucin glycoproteins
Author(s) -
Afdhal Nezam H.,
Gong Dahai,
Niu Niu,
Turner Bradley,
Lamont J. Thomas,
Offner Gwynneth D.
Publication year - 1993
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840170425
Subject(s) - glycoprotein , medicine , cholesterol , prostaglandin , concanavalin a , endocrinology , mucin , gallbladder , hepatology , lipidology , chemistry , biology , biochemistry , in vitro
The aims of this study were to examine the effect of exogenous prostaglandin on mucin secretion and to determine the role of nonmucin glycoproteins on gallstone formation in the prairie dog model of cholesterol cholelithiasis. The concentration of total glycoprotein and nonmucin glycoproteins was measured in gallbladder bile from four groups of prairie dogs fed a control diet or a diet supplemented with 1.2% cholesterol with or without simultaneous subcutaneous administration of prostaglandin E 1 . Cholesterol feeding resulted in an increased concentration of concanavalin‐A binding–proteins in gallbladder bile associated with an increase in pronucleating activity in vitro . Treatment with prostaglandin E 1 and cholesterol feeding was associated with a significant increase in the incidence of cholesterol gallstone formation. Prostaglandin E 1 treatment in the cholesterol‐fed animals increased biliary concentrations of total glycoprotein and concanavalin‐A–binding glycoproteins. Therefore the increased biliary glycoprotein level in cholesterolfed, prostaglandin E 1 ‐treated prairie dogs, which reflects higher levels of mucin and nonmucin glycoproteins, appears to be an important factor in gallstone formation. (H EPATOLOGY 1993;17:693–700.)