Premium
Dissection of compartments in rat hepatocytes involved in the intracellular trafficking of high‐density lipoprotein particles or their selectively internalized cholesteryl esters
Author(s) -
Jäckle Stefan,
Rinninger Franz,
Lorenzen Thomas,
Greten Heiner,
Windler Eberhard
Publication year - 1993
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840170316
Subject(s) - endosome , lipoprotein , cholesteryl ester , apolipoprotein b , apolipoprotein e , chemistry , intermediate density lipoprotein , high density lipoprotein , endocytosis , low density lipoprotein , intracellular , lrp1 , biochemistry , hepatocyte , cholesterol , biophysics , very low density lipoprotein , ldl receptor , biology , receptor , in vitro , medicine , disease
Abstract The trafficking of apolipoprotein E–deficient highdensity lipoprotein particles and of their component cholesteryl esters in rat hepatocytes was studied. Human high‐density lipoprotein 3, labeled with two nondegradable, intracellularly trapped tracers in their apolipoprotein A–I and their cholesteryl esters, were injected into rats, and five subcellular hepatocytic fractions were isolated at various time intervals. In control experiments with homologous lipoproteins, doubly labeled rat high‐density lipoproteins depleted of apolipoprotein E were used. In endosomes and lysosomes the two labels were recovered at near unity, indicating that high‐density lipoproteins are endocytosed as particles, transported to early and late endosomes and finally subjected to lysosomal degradation. No significant amounts of label were found in receptorrecycling endosomes. In contrast to label of those of low‐density lipoproteins, label of component protein and cholesteryl esters of high‐density lipoproteins from isolated endosomes floated at different densities in gradient ultracentrifugation, indicating early disintegration of high‐density lipoprotein particles. In contrast to the endocytic organelles, in the whole liver, label of high‐density lipoprotein–associated cholesteryl esters exceeded the label of high‐density lipoprotein–associated apolipoprotein A‐I twofold to threefold. This finding is compatible with selective uptake of high‐density lipoprotein cholesteryl esters in addition to uptake of high‐density lipoprotein particles. The excess cholesteryl esters accumulated in a nonendosomal fraction, whose major proteins differed from the integral proteins of endosomes. These data suggest two distinct intracellular routes of hepatocytic high‐density lipoprotein trafficking in vivo . Highdensity lipoproteins free of apolipoprotein E are internalized intact by hepatocytes, are predominantly transported to early and late endosomes and are finally subjected to lysosomal degradation. High‐density lipo‐protein particles do not undergo retroendocytosis in hepatocytes. In addition, high‐density lipoprotein–associated cholesteryl esters can be taken up by hepatocytes selectively. They, however, accumulate in a nonendosomal, nonlysosomal compartment. (H EPATOLOGY 1993;17:455–465.)