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Human cholecystitis is associated with increased gallbladder prostaglandin I 2 and prostaglandin E 2 synthesis
Author(s) -
Myers Stuart I.,
Bartula Lori
Publication year - 1992
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840160512
Subject(s) - microsome , gallbladder , prostaglandin , medicine , prostaglandin e2 , cholecystectomy , arachidonic acid , prostaglandin e , endocrinology , cholecystitis , eicosanoid , chemistry , biochemistry , enzyme
Microsomal prostanoid synthesis was compared in normal gallbladders removed during organ donation and inflamed gallbladders removed at cholecystectomy. Normal human gallbladder microsomes demonstrated low rates of conversion of [ 14 C]arachidonic acid to total labeled prostanoids, which increased during 1 to 30 min of incubation. Normal human gallbladder microsomes converted labeled substrate to all primary prostaglandins without demonstration of a major product. Inflamed human gallbladder microsomes increased the rate of conversion of [ 14 C]arachidonic acid to total labeled prostanoids two or three times over the levels demonstrated by normal gallbladder microsomes at all times of incubation (p < 0.01). The main prostanoids synthesized by the inflamed human gallbladder microsomes were prostaglandin E 2 and 6‐keto‐prostaglandin F 1α , which were increased four times over the levels demonstrated by normal gallbladder microsomes (p < 0.01). These data showed that inflammation of the human gallbladder was associated with increased synthesis of gallbladder 6‐keto‐prostaglandin F 1α , and prostaglandin E 2 . (H EPATOLOGY 1992;16:1176–1179.)