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Localization of cytochrome P‐450 gene expression in normal and diseased human liver by in situ hybridization of wax‐embedded archival material
Author(s) -
Palmer Colin N. A.,
Coates Philip J.,
Davies Susan E.,
Shephard Elizabeth A.,
Phillips Ian R.
Publication year - 1992
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840160311
Subject(s) - microbiology and biotechnology , cyp3a , biology , gene , in situ hybridization , acinus , gene expression , cytochrome , cytochrome p450 , parenchyma , cyp1a2 , biochemistry , enzyme , pancreas , botany
The localization of the expression of several cytochrome P‐450 genes in normal and diseased human liver was investigated by in situ hybridization of formalin‐fixed, paraffin wax–embedded archival tissue samples with 35 S‐labeled antisense RNA probes. The results demonstrated that genes coding for members of the cytochrome P‐450 3A subfamily (CYP3A) were preferentially expressed in hepatocytes in acinar zone 3 (the centrilobular region), whereas genes coding for CYP1A2, CYP2A, 2B and 2C were expressed uniformly throughout the liver acinus. In cirrhotic livers, CYP2A and 2B genes (and to a lesser extent, CYP3A genes) were highly expressed in isolated hepatocytes located at the junction of parenchyma with fibrous septa. The cause and significance of the position‐dependent expression of specific cytochrome P‐450 genes in normal and diseased human liver are discussed. (H EPATOLOGY 1992;16:682–687.)