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Ultrastructural identification of light microscopic giant mitochondria in alcoholic liver disease
Author(s) -
Inagaki Takao,
Kobayashi Susumu,
Ozeki Norio,
Suzuki Masahiro,
Fukuzawa Yoshitaka,
Shimizu Kazuhito,
Kato Katsuhisa,
Kato Katsumi
Publication year - 1992
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840150110
Subject(s) - ultrastructure , alcoholic liver disease , identification (biology) , mitochondrion , pathology , biology , medicine , chemistry , microbiology and biotechnology , botany , cirrhosis
Ultrastructural identification of light microscopic giant mitochondria was performed on the same specimens for light and electron microscopic observations. The liver tissue specimens were fixed in OsO 4 , embedded in epoxy resin, cut 4 μm thick and stained with polychrome. At the beginning of the study a light microscopic observation was made, and a microphotograph was taken. The identification of light microscopic giant mitochondria by conventional microscopy was identified by the occupation rate in liver cells, the negative findings of stainability and the morphological consistency (round, cigar‐shaped and granular). The specimens were subsequently embedded again in epoxy resin and cut into ultrathin sections of 400 Å. A transillumination electron microscope was used for the observation, and ultrastructural images of light microscopic giant mitochondria revealed that they were crystalloid bodies with a crystalline latticelike structure. The occupation rates within liver cells and the morphological shapes of the crystalloid bodies corresponded with those of light microscopic giant mitochondria. The light microscopic giant mitochondria obviously had different features from those of electron microscopic giant mitochondria and Mallory bodies (Yokoo's type II), although Mallory bodies showed the same staining properties as light microscopic giant mitochondria. (H EPATOLOGY 1992; 15:46–53).