Premium
Decreased hepatic glycogen content and accelerated response to starvation in rats with carbon tetrachloride–induced cirrhosis
Author(s) -
Krahenbuhl Stephan,
Weber Fred L.,
Brass Eric P.
Publication year - 1991
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840140638
Subject(s) - carbon tetrachloride , cirrhosis , starvation , glycogen , medicine , chemistry , endocrinology , carbon tetrachloride poisoning , organic chemistry
Glucose homeostasis and fatty acid metabolism are abnormal in patients with cirrhosis. To assess the metabolic response to starvation in an animal model of cirrhosis, glycogen and fuel metabolism were characterized in rats with CCl 4 ‐induced cirrhosis studied 2 wk after 10 weekly doses of CCl 4 . Plasma concentrations of glucose and β‐hydroxybutyrate were not different between fed CCl 4 ‐treated and control rats, but plasma nonesterified fatty acid concentrations were higher in cirrhotic animals (0.25 ± 0.01 vs. 0.39 ± 0.04 mmol/L; p < 0.05). After 12 hr of starvation, the plasma nonesterified fatty acid concentration had reached 0.58 ± 0.04 mmol/L in CCl 4 ‐treated rats, compared with 0.38 ± 0.04 mmol/L in control rats (p < 0.05). The redistribution of the hepatic carnitine pool toward acylcarnitines, which is characteristic of starvation, was complete after fasting for 12 hr in the CCl 4 ‐treated rats, compared with the 24 hr required in control rats. In fed cirrhotic rats, liver glycogen content per gram liver was decreased by 64% compared with control rats (30.0 ± 5.1 vs. 10.8 ± 1.1 mg/gm liver wet wt; p < 0.05). After 12‐hr fasting, hepatic glycogen content had fallen to 14.3 ± 3.9 and 4.8 ± 0.4 mg/gm liver wet wt (p < 0.05) in control and cirrhotic animals, respectively. To further characterize the status of glycogen metabolism in cirrhotic livers, activities of glycogen synthase and glycogen phosphorylase were determined. Hepatic active and total glycogen phosphorylase activities normalized to hepatocellular content were unaffected by CCl 4 treatment, whereas total glycogen synthase activity was increased by 45%. In conclusion, hepatic glycogen content in fed animals with CCl 4 ‐induced cirrhosis is decreased, and cirrhotic animals have accelerated transition from the fed to the fasted state, as evidenced by hepatic glycogen depletion, increased plasma nonesterified fatty acid concentrations and redistribution of the hepatic carnitine pool. Rats with CCl 4 ‐induced liver cirrhosis provide a useful animal model to study changes in energy metabolism induced by chronic liver disease. (H EPATOLOGY 1991;14:1189–1195.)