Premium
Immunohistochemical detection of transforming growth factor‐β 1 in fibrotic liver diseases
Author(s) -
Nagy Peter,
Schaff Zsuzsa,
Lapis Károly
Publication year - 1991
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840140211
Subject(s) - cirrhosis , fibrosis , immunohistochemistry , pathology , polyclonal antibodies , transforming growth factor , liver biopsy , staining , transforming growth factor beta , gdf15 , chronic liver disease , growth factor , liver disease , hepatitis , antibody , medicine , biopsy , immunology , receptor
Transforming growth factor‐β 1 was localized by means of immunohistochemical reaction in liver biopsy specimens taken from patients having different chronic liver diseases with extending fibrosis. Two polyclonal antibodies that were produced in rabbits were directed against the amino terminal of transforming growth factor‐β 1 . Staining by anti‐CC(1–30) was primarily extracellular and located in the portal and periportal fibrotic areas of all seven cases with chronic active hepatitis. No staining was noted in the four chronic persistent cases studied. A strong reaction was seen with the antibody in nine of the ten cirrhotic samples, whereas it was negative in one inactive cirrhosis case and in all five cases with normal liver histological findings. No positive staining could be detected by the anti‐LC(1–30) in any of the liver tissues. Detection of transforming growth factor‐β 1 in active liver diseases at the site of fibrosis suggests that transforming growth factor‐β 1 might have a role in the process and progression of fibrosis during the development of the disease. (HEPATOLOGY 1991;14:269–273.)