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Regulation of fatty‐acid metabolism by pancreatic hormones in cultured human hepatocytes
Author(s) -
Vons Corinne,
Pegorier JeanPaul,
Girard Jean,
Kohl Claude,
Ivanov MarieAgnès,
Franco Dominique
Publication year - 1991
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840130620
Subject(s) - hormone , metabolism , fatty acid metabolism , fatty acid , endocrinology , biochemistry , medicine , biology , chemistry
The effects of pancreatic hormones and cyclic AMP on long‐chain fatty‐acid metabolism were investigated in human hepatocytes isolated from 12 liver biopsy specimens and cultured for 4 days in an insulin‐free medium. Glucagon (10 −6 mol/L) increased endogenous ketone body production by 150%. This resulted from alterations in the partition of long‐chain fatty acids from esterification toward oxidation. Glucagon or cyclic AMP enhanced ( 14 C) oleate oxidation (basal = 45.8% ± 5.0%; glucagon = 66.8% ± 5.3%; cyclic AMP = 67.6% ± 5.0% of metabolized oleate) at the expense of oleate esterification. Insulin (10 −7 mol/L) antagonized the glucagon‐induced oleate oxidation. After 24 hr in basal culture conditions, the rate of lipogenesis decreased to the same low rate as in glucagon‐treated cells. The presence of insulin did not restore a high rate of lipogenesis. These results are the first direct evidence of a control of ketone body production by glucagon in the human liver. (H EPATOLOGY 1991;13:1126–1130.)