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Fucosyltransferases: Differential plasma and tissue alterations in hepatocellular carcinoma and cirrhosis
Author(s) -
Hutchinson Winston L.,
Du MingQing,
Johnson Philip J.,
Williams Roger
Publication year - 1991
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840130412
Subject(s) - hepatocellular carcinoma , cirrhosis , medicine , liver tissue , endocrinology , alpha (finance) , carcinoma , biology , construct validity , nursing , patient satisfaction
To determine whether abnormal metabolism of l‐fucose in hepatocellular carcinoma is accompanied by alterations in the activities of fucosyltransferases, the latter were determined in plasma and liver tissue of patients with this disease and in cirrhotic and normal subjects. Activities of α‐2/α‐3 and α‐6‐l ‐fucosyltransferases were all significantly greater in plasma from patients with hepatocellular carcinoma than in plasma from cirrhotic patients or normal subjects (p < 0.025). The activity of each enzyme was dependent, to a similar extent, on Mn 2+ , Mg 2+ and triton X‐10, irrespective of the source, and all displayed pH optimums in the range of 7.5 to 8.0. In contrast, activities of α‐2/α‐3 fucosyltransferases were significantly lower (p < 0.025) in homogenates prepared from tumorous liver tissue than in that prepared from nontumorous tissue from hepatocellular carcinoma and cirrhotic patients, whereas for the α‐6 enzyme the situation was reversed (typically, tumor tissue levels were 5 pmol/hr/mg; in nontumor tissue they were 2 pmol/hr/mg). Activities of galactosyl and mannosyltransferase in tumor tissue were greater in all cases than in nontumor cirrhotic tissue. Plasma fucosyltransferases are specifically elevated in hepatocellular carcinoma but different mechanisms appear to underlie the changes seen for α‐2/α‐3 and α‐6‐l‐fucosyltransferases. (H EPATOLOGY 1991;13:683–688.)